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Re: Molecular clock calibration

Subject: Re: Molecular clock calibration
From: Philip Deitiker
Date: Sat, 10 Dec 2005 18:49:31 GMT
Newsgroups: sci.anthropology.paleo
In sci.anthropology.paleo message 
news:1134227239.079556.41950@xxxxxxxxxxxxxxxxxxxxxxxxxxxx by
"johnwl4@xxxxxxx" <johnwl4@xxxxxxx>  . . . : 

>     While reading about the spread of people through Oceania, I
>     was 
> referred back to an opinion by Thomas Parsons.   He says the
> variation between sequences of mothers and daughters is much
> higher than would be predicted from the molecular clock.   He
> refers to 'hot spots'  cause this high variation.   So, how was
> this resolved?   Does the molecular clock just refer to an
> average?   How does linkage disequilibrium come into this?
>     Incidentally, why the phrase, "linkage disequilibrium"  ?   
>     Seems 
> confusing to me, as if two sites were not linked.

On mtDNA everything is linked, LD is 100%. Its only recurrent 
mutations that present an alternative point of view. When there is 
region specific recurrency the one hypothesis is recombination. I 
would point out that recombination is probably of the abortive 
nature, gene conversion, and is swapping common variants between 
mtDNA types. One of the principle issues however it hypervariability 
in hypervariable sites. sites which are so prone to mutation that 
they are best not considered in clocking. There are in fact 100s of 
molecular clocks operating simultaneous on mtDNA, fairly we can been 
the positions into about 10 clockable speeds, and each speed is 
scattered around the mtDNA. When one talks about mtDNA mutation then 
it is an average of those rates over time. Within one or two clock 
ticks ones conclusions can be very poor. Even so calibration is 
between 18 and 35ky per HVR1 'tick' and this does not consider rate 
variation on top of that. relative Error = Deviation/Mean. SE = SD 
(Time)/(N^0.5), Mean = N(number of accumulated mutations) * cycle 
length. 
  Increasing the number of mutational differences between two 
divergent types increase the SE but never beyond the confidence of 
the calibration confidence. I have estimated that gene conversion 
could be altering short term estimates by 10%, over long term it may 
be a primary cause of transitional fading in a population. For 
example the observation of transition to tranversion rates between 
chimps and humans is about 2.5:1 for HVR1, but if one looks at 
individuals chimps, gorilla or human trees with lots of branches the 
transition to transversion rate is 15:1 in humans (and monkeys) and 
10.8:1 in chimps and gorilla. The faster evolving sites tend to be 
the places where the recurrent mutations are occurring, but once a 
mutation is common in a bivariant population, then that mutation 
appears to become recurrent. The anti-recombination folks have failed 
to explain why that is. 





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